By Peter Kapusta,Michael Wahl,Rainer Erdmann
This quantity makes a speciality of Time-Correlated unmarried Photon Counting (TCSPC), a strong device permitting luminescence lifetime measurements to be made with excessive temporal answer, even on unmarried molecules. Combining spectrum and lifelong offers a “fingerprint” for choosing such molecules within the presence of a historical past. Used including confocal detection, this enables single-molecule spectroscopy and microscopy as well as ensemble measurements, commencing up an immense variety of sizzling lifestyles technological know-how purposes equivalent to fluorescence lifetime imaging (FLIM) and size of Förster Resonant power move (FRET) for the research of protein folding and interplay. numerous technology-related chapters current either the fundamentals and present state of the art, particularly of TCSPC electronics, photon detectors and lasers. the remainder chapters hide a wide diversity of purposes and methodologies for experiments and information research, together with the existence sciences, disorder facilities in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new suggestions bobbing up from the combo of vintage TCSPC and fluorescence lifetime with equipment in accordance with depth fluctuation symbolize a very targeted highlight.
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Extra resources for Advanced Photon Counting: Applications, Methods, Instrumentation (Springer Series on Fluorescence)
Advanced Photon Counting: Applications, Methods, Instrumentation (Springer Series on Fluorescence) by Peter Kapusta,Michael Wahl,Rainer Erdmann